RayBio® Human Myeloperoxidase ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human Myeloperoxidase ELISA Kit
Human Myeloperoxidase ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.26 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.26 ng/ml - 75 ng/ml |
| Specificity | This ELISA antibody pair detects human MPO. Other species not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P05164 |
| Gene Id | 4353 |
| Gene Symbols | MPO |
| Protein Name / Synonyms | Myeloperoxidase (MPO) (EC 1.11.2.2) [Cleaved into: Myeloperoxida |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Myeloperoxidase ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Myeloperoxidase, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Agonist signaling drives neutrophil subpopulations to promote/inhibit colorectal cancer liver metastasis
- Species:
- Human
- Sample Type:
- Other / Unspecified (cell CM from CRC, non-tumor cells)
- Year:
- 2025
- Journal Name:
- Nature Communications
- PubMed ID:
- 41408063
Case Report: Anti-platelet factor 4 -mediated immunothrombosis in a patient with ANCA vasculitis - a shared mechanism of NETosis
- Species:
- Human
- Sample Type:
- Serum (ANCA vasculitis patient)
- Year:
- 2025
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 40276517
Single-cell Sequencing Reveals Neutrophil Extracellular Traps in Association with Endotheliopathy and Immunothrombosis in Hepatitis B Virus-related Acute-on-chronic Liver Failure
- Species:
- Human
- Sample Type:
- Plasma (HBV-related ACLF patients)
- Year:
- 2026
- Journal Name:
- Journal of clinical and translational hepatology
- PubMed ID:
- 42181839
Quantification of intra-amniotic inflammation in late preterm prelabour rupture of membranes
- Species:
- Human
- Sample Type:
- Other Body Fluid (amniotic fluid)
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 40295647
Recharging Healthcare Professionals: A Randomized Controlled Trial on the Impact of Automated Massage Chairs on Depression, Anxiety, Stress, Musculoskeletal Pain, and Biochemical Markers
- Species:
- Human
- Sample Type:
- Serum (healthcare professionals, massage chair RCT)
- Year:
- 2025
- Journal Name:
- Health science reports
- PubMed ID:
- 40959182
