Human PDGFRA (Platelet-derived growth factor receptor alpha) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	PDGFRA
Gene ID:	5156
Uniprot ID:	PGFRA_HUMAN
Specificity:	This kit recognizes Human PDGFR Alpha in samples. No significant cross-reactivity or interference between Human PDGFR Alpha and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
Post Translational Modifications	N-glycosylated. Ubiquitinated, leading to its internalization and degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-731 and Tyr-742 is important for interaction with PIK3R1. Phosphorylation at Tyr-720 and Tyr-754 is important for interaction with PTPN11. Phosphorylation at Tyr-762 is important for interaction with CRK. Phosphorylation at Tyr-572 and Tyr-574 is important for interaction with SRC and SRC family members. Phosphorylation at Tyr-988 and Tyr-1018 is important for interaction with PLCG1.
Function	Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades.the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Protein Name	Platelet-Derived Growth Factor Receptor Alpha Pdgf-R-Alpha Pdgfr-Alpha Alpha Platelet-Derived Growth Factor Receptor Alpha-Type Platelet-Derived Growth Factor Receptor Cd140 Antigen-Like Family Member A Cd140a Antigen Platelet-Derived Growth Factor Alpha Receptor Platelet-Derived Growth Factor Receptor 2 Pdgfr-2 Cd Antigen Cd140a
Database Links	Reactome: R-HSA-1257604 Reactome: R-HSA-186763 Reactome: R-HSA-186797 Reactome: R-HSA-2219530 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9674396 Reactome: R-HSA-9674401 Reactome: R-HSA-9674403 Reactome: R-HSA-9674404 Reactome: R-HSA-9674428
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Cilium Golgi Apparatus
Alternative CLIA Names	Platelet-Derived Growth Factor Receptor Alpha CLIA kit Pdgf-R-Alpha CLIA kit Pdgfr-Alpha CLIA kit Alpha Platelet-Derived Growth Factor Receptor CLIA kit Alpha-Type Platelet-Derived Growth Factor Receptor CLIA kit Cd140 Antigen-Like Family Member A CLIA kit Cd140a Antigen CLIA kit Platelet-Derived Growth Factor Alpha Receptor CLIA kit Platelet-Derived Growth Factor Receptor 2 CLIA kit Pdgfr-2 CLIA kit Cd Antigen Cd140a CLIA kit PDGFRA CLIA kit PDGFR2 CLIA kit RHEPDGFRA CLIA kit
Specificity	This kit recognizes Human PDGFRα in samples. No significant cross-reactivity or interference between Human PDGFRα and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-101	95
EDTA plasma(n=8)	93-111	101
Cell culture media(n=8)	84-97	91

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	105.27	189.69	704.85	97.94	173.05	661.86
Standard deviation	10.08	21.06	62.66	11.87	19.95	58.24
CV (%)	9.58	11.10	8.89	12.12	11.53	8.80

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance