General Info
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description: | This human PRSS2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | PRSS2 |
| Gene ID: | 5645 |
| Uniprot ID: | TRY2_HUMAN |
| Specificity: | This kit recognizes Human PRSS2 in samples. No significant cross-reactivity or interference between Human PRSS2 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Tissue Specificity | Expressed in Paneth cells, at the base of small intestinal crypts. |
| Post Translational Modifications | Sulfated on tyrosine. Sulfation at Tyr-154 increases selectivity towards basic versus apolar residues at the P2' position of inhibitors that bind in a substrate-like fashion. Although the increase in selectivity is relatively small, it may facilitate digestion of a broader range of dietary proteins. |
| Function | In the ileum, may be involved in defensin processing, including DEFA5. |
| Protein Name | Trypsin-2Anionic TrypsinogenSerine Protease 2Trypsin Ii |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1462054Reactome: R-HSA-1592389Reactome: R-HSA-6798695Reactome: R-HSA-6803157 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative CLIA Names | Trypsin-2 CLIA kitAnionic Trypsinogen CLIA kitSerine Protease 2 CLIA kitTrypsin Ii CLIA kitPRSS2 CLIA kitTRY2 CLIA kitTRYP2 CLIA kit |
| Specificity | This kit recognizes Human PRSS2 in samples. No significant cross-reactivity or interference between Human PRSS2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-105 | 97 |
| EDTA plasma(n=8) | 96-110 | 104 |
| Cell culture media(n=8) | 92-106 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.51 | 1.35 | 3.58 | 0.48 | 1.24 | 3.43 |
| Standard deviation | 0.06 | 0.13 | 0.37 | 0.06 | 0.10 | 0.39 |
| CV (%) | 11.76 | 9.63 | 10.34 | 12.50 | 8.06 | 11.37 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance