Human Pyruvate Kinase Muscle CLIA Kit

Human Pyruvate Kinase, Muscle CLIA Kit is a highly sensitive Chemiluminescent Immunoassay (CLIA) for meauring Pyruvate Kinase, Muscle in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests.
This assay recognizes Human PKM2 in samples. No significant cross-reactivity or interference between Human PKM2 and analogues was observed. Our Pyruvate Kinase, Muscle CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PKM2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PKM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PKM2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PKM2. You can calculate the concentration of Human PKM2 in the samples by comparing the RLU value of the samples to the standard curve.

Small volumes of Pyruvate Kinase Muscle clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8425828 is a CLIA kit targeting Pyruvate Kinase Muscle. Also known as Human Pyruvate Kinase Muscle CLIA Kit. Reactive in Human.