RayBio® C-Series Human Receptor Antibody Array 1 Kit. Detects 40 human cytokine receptor proteins. Suitable for all liquid sample types.
Human Receptor Array C1
Human Receptor Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 40 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
4-1BB (TNFRSF9/CD137)
| ALCAM (CD166)
| CD80 (B7-1)
| BCMA (TNFRSF17)
| CD14
|
CD30 (TNFRSF8)
| CD40 Ligand (TNFSF5)
| CEACAM-1
| DR6 (TNFRSF21)
| Dtk
|
Endoglin (CD105)
| ErbB3
| E-Selectin
| Fas (TNFRSF6/Apo-1)
| Flt-3 Ligand
|
GITR (TNFRSF18)
| HVEM (TNFRSF14)
| ICAM-3 (CD50)
| IL-1 R4 (ST2)
| IL-1 R1
|
IL-10 R beta
| IL-17 RA
| IL-2 R gamma (Common gamma Chain)
| IL-21 R
| LIMPII
|
Lipocalin-2 (NGAL)
| L-Selectin (CD62L)
| LYVE-1
| MICA
| MICB
|
NRG1-beta 1 (HRG1-beta 1)
| PDGF R beta
| PECAM-1 (CD31)
| RAGE
| TIM-1 (KIM-1)
|
TRAIL R3 (TNFRSF10C)
| Trappin-2
| uPAR
| VCAM-1 (CD106)
| XEDAR
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Receptor Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.