Human RETN (Resistin) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	RETN
Gene ID:	56729
Uniprot ID:	RETN_HUMAN
Specificity:	This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in white adipose tissue (at protein level). Widely expressed, with particularly strong expression in lung, bone marrow, breast and peripheral blood. Expressed strongly in bone marrow and at lower levels in lung, but not detected in other tissues. Isoform 2 is detected in adipose tissue, bone marrow, brain, lung, peripheral blood, placenta and thymus.
Function	Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Promotes chemotaxis in myeloid cells.
Protein Name	Resistin Adipose Tissue-Specific Secretory Factor Adsf C/Ebp-Epsilon-Regulated Myeloid-Specific Secreted Cysteine-Rich Protein Cysteine-Rich Secreted Protein A12-Alpha-Like 2 Cysteine-Rich Secreted Protein Fizz3
Database Links	Reactome: R-HSA-6798695 Reactome: R-HSA-9615017
Cellular Localisation	Secreted
Alternative CLIA Names	Resistin CLIA kit Adipose Tissue-Specific Secretory Factor CLIA kit Adsf CLIA kit C/Ebp-Epsilon-Regulated Myeloid-Specific Secreted Cysteine-Rich Protein CLIA kit Cysteine-Rich Secreted Protein A12-Alpha-Like 2 CLIA kit Cysteine-Rich Secreted Protein Fizz3 CLIA kit RETN CLIA kit FIZZ3 CLIA kit HXCP1 CLIA kit RSTN CLIA kit UNQ407 CLIA kit PRO1199 CLIA kit
Specificity	This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-105	96
EDTA plasma(n=8)	97-113	104
Cell culture media(n=8)	86-100	92

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	43.23	66.99	375.61	45.42	64.95	344.70
Standard deviation	5.26	7.31	24.64	4.88	7.20	38.78
CV (%)	12.17	10.91	6.56	10.74	11.09	11.25

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance