AAH-TH17-1-8 | Human Th1/Th2/Th17 Array C1 Clinisciences

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CD30 (TNFRSF8) Gene Symbol: CD30 Gene ID: 943 Accession #: P28908 ELISA Kit: RayBio Human CD30 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Gene ID: 8784 Accession #: Q9Y5U5 ELISA Kit: RayBio Human GITR ELISA
GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-1 R1 Gene Symbol: IL1R1 Gene ID: 3554 Accession #: P14778 ELISA Kit: RayBio Human IL-1 sRI ELISA	IL-1 R2 Gene Symbol: IL1R2 Gene ID: 7850 Accession #: P27930 ELISA Kit: RayBio Human IL-1 sRII ELISA
IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-6 R Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA
IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552
IL-17F Gene Symbol: IL17F Gene ID: 112744 Accession #: Q96PD4 ELISA Kit: RayBio Human IL-17F ELISA	IL-17 RA Gene Symbol: IL17RA Gene ID: 23765 Accession #: Q96F46 ELISA Kit: RayBio Human IL-17 R ELISA	IL-21 Gene Symbol: IL21 Gene ID: 59067 Accession #: Q9HBE4 ELISA Kit: RayBio Human IL-21 ELISA	IL-21 R Gene Symbol: IL21R Gene ID: 50615 Accession #: Q9HBE5 ELISA Kit: RayBio Human IL-21 R ELISA	IL-22 Gene Symbol: IL22 Gene ID: 50616 Accession #: Q9GZX6 ELISA Kit: RayBio Human IL-22 ELISA
IL-23 Gene Symbol: IL23A Gene ID: 51561 Accession #: Q9NPF7 ELISA Kit: RayBio Human IL-23 ELISA	IL-28A (IFN-lambda 2) Gene Symbol: IFNL2 Gene ID: 282616 Accession #: Q8IZJ0 ELISA Kit: RayBio Human IL-28A ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA	gp130 Gene Symbol: Il6st Gene ID: 3572 Accession #: P40189 ELISA Kit: RayBio Human sgp130 ELISA	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA
TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Gene ID: 8600 Accession #: O14788

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Th1/Th2/Th17 Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	34
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Research Area	T Helper Cells, Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Human Th1/Th2/Th17 Array C1

Cat# AAH-TH17-1-8

Marca : RayBiotech

Human Th1/Th2/Th17 Array C1

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

documents

citations

Human monocytes differentiate into tumor-associated macrophages upon SKOV3 cells coculture and/or lysophosphatidic acid stimulation

Linalool Exhibits Cytotoxic Effects by Activating Antitumor Immunity

Usefulness of the cytokines expression of Th1/Th2/Th17 and urinary CD80 excretion in adult-onset minimal change disease

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