Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> IgG3 (HRP)

IgG3 (HRP)

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Cat# I1904-85H-1mg

Size : 1mg

Marca : US Biological


I1904-85H IgG3 (HRP)

Clone Type
Polyclonal
Host
rabbit
Source
mouse
Isotype
IgG
Grade
Affinity Purified
Applications
DB E IHC WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for Western or Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:31,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1mg/ml (UV280)(after reconstitution)|Form: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.15M sodium chloride, 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Mouse IgG3 heavy chain.|Specificity: Reconizes Mouse IgG3. Assay by IEP resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgG3 heavy chain.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.15M sodium chloride, 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Reconizes Mouse IgG3. Assay by IEP resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
References
1. Farr & Nakane, J. Immunol. Methods, 47:129-144, 1981.