IL1 beta antibody
Cat# GTX22105-100ug
Size : 100ug
Marca : Genetex
Contatta il distributore locale :
Telefono : +1 850 650 7790
Host | Rabbit |
|---|---|
Clonality | Polyclonal |
Isotype | IgG |
Application | WB, IHC-P, IHC-Fr, FACS, IP, ELISA, Neutralizing/Inhibition |
Reactivity | Human, Dog |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX22105 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:1:100-1:200 |
| IHC-Fr | 1:1:100-1:200 |
| FACS | Assay dependent |
| IP | 1:400-1:800 |
| ELISA | 1:500-1:2000 |
| Neutralizing/Inhibition | 1:100 |
Not tested in other applications.
Calculated MW
31 kDa. ( Note )
Product Note
Primarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions.The antiserum does not recognize human IL-1alpha.
PROPERTIES
Form
Liquid
Buffer
20mM Potassium Phosphate, 150mM NaCl
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-1s produced in E.coli. The MW of the recombinant 153 aa IL-1s was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1s.
Purification
Purified by DEAE chromatography.
From serum
From serum
Conjugation
Unconjugated
RRID
AB_368268
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
Cellular Localization
Cytoplasm, cytosol,Lysosome,Secreted
Background
Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
Database
DATA IMAGES
| | GTX22105 WB ImageGTX22105 was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1beta by Western blot. Lane 1 and 2 were loaded with 2.5 μg and 500 ng of dog IL-1beta respectively. |
| | GTX22105 IHC-P ImageIHC-P analysis of medullary lymph node using IL1 beta antibody. Positive staining in the cytoplasm of circulating macrophages. Negative Control (far right) normal rabbit IgG. |
| | GTX22105 WB Image WB analysis of recombinant human IL1 beta protein using GTX22105 IL1 beta antibody. |
| | GTX22105 IHC-P ImageIHC-P analysis of human medullary lymph node tissue using GTX22105 IL1 beta antibody. |
| | GTX22105 WB ImageThe data was published in the journal Int J Mol Sci in 2019. PMID: 30987270 |
Application Reference
Sgrignoli MR et al. Stem Cell Res 2019; 39 : 101525 Reduction in the inflammatory markers CD4, IL-1, IL-6 and TNF帢 in dogs with keratoconjunctivitis sicca treated topically with mesenchymal stem cells.
Application : IHC-P
Reactivity : Dog
Lee YC et al. Int J Mol Sci 2019; 20 (7) Essential Role of Visfatin in Lipopolysaccharide and Colon Ascendens Stent Peritonitis-Induced Acute Lung Injury.
Application : WB
Reactivity : Human
Chu Y et al. Int J Mol Sci 2019; 20 (1) Protective Effects and Mechanisms of N-Phenethyl Caffeamide from UVA-Induced Skin Damage in Human Epidermal Keratinocytes through Nrf2/HO-1 Regulation.
Application : WB
Reactivity : Human
Lai WY et al. Int J Oral Sci 2014; 6 (2) : 34547 An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements.
Application : WB
REVIEW
Application Reference
Sgrignoli MR et al. Stem Cell Res 2019; 39:101525 Reduction in the inflammatory markers CD4, IL-1, IL-6 and TNF帢 in dogs with keratoconjunctivitis sicca treated topically with mesenchymal stem cells.
Application : IHC-P
Reactivity : Dog
Lee YC et al. Int J Mol Sci 2019; 20 (7) Essential Role of Visfatin in Lipopolysaccharide and Colon Ascendens Stent Peritonitis-Induced Acute Lung Injury.
Application : WB
Reactivity : Human
Chu Y et al. Int J Mol Sci 2019; 20 (1) Protective Effects and Mechanisms of N-Phenethyl Caffeamide from UVA-Induced Skin Damage in Human Epidermal Keratinocytes through Nrf2/HO-1 Regulation.
Application : WB
Reactivity : Human
Lai WY et al. Int J Oral Sci 2014; 6 (2):34547 An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements.
Application : WB
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