Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

IPTG [367-93-1]

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Cat# NB-64-28601-500mg

Size : 500mg

Marca : Neo Biotech

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IPTG

(Synonyms: Isopropyl β-D-thiogalactoside) Copy Product Info

Synonyms: Isopropyl β-D-thiogalactoside

Catalog No. T4336 Copy Product Info

Purity: 99.96%

IPTG (Isopropyl β-D-thiogalactoside) is a synthetic, non-metabolizable galactose analog. IPTG can trigger the transcription process of the lactose operon, thereby inducing the expression of corresponding proteins encoded by downstream genes of the lactose operon.

Cas No. 367-93-1

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.96%

Color:White

Product Information

Bioactivity

Description	IPTG (Isopropyl β-D-thiogalactoside) is a synthetic, non-metabolizable galactose analog. IPTG can trigger the transcription process of the lactose operon, thereby inducing the expression of corresponding proteins encoded by downstream genes of the lactose operon.
In vitro	Methods: Add IPTG to E. coli carrying the PLtetO-1-luc reporter plasmid at a concentration gradient (0.01–5 mM). Culture until the logarithmic growth phase, then detect luciferase activity and calculate the fold change in expression levels. Results: Plac/ara-1 exhibits an IPTG-induced regulatory range of up to 1800-fold. [1]
Synonyms	Isopropyl β-D-thiogalactoside
Kinase Assay	For RNA interference (RNAi), A549 cells are plated at 1,500 cells per well in 96-well plates, allowed to adhere for 24 hours, and transfected with 25 nM siRNA oligonucleotide using Dharmafect 4. Transfected cells are treated with the indicated concentrations of GNE-617 (0.1, 1 , 10 , 100 , and 1000 nM) for 72 hours and viability is evaluated with CellTiter-Glo. Lysates for detection of NAPRT1 protein are collected 72 hours after transfection of 1 million A549 cells in 10 cm dishes. For NAPRT1 re-expression, RERF-LC-MS cells are transfected with pCMV6-AC.NAPRT1 and empty vector pCMV6-AC using Amaxa Nucleofector technology and selected with Geneticin

Chemical Properties

Molecular Weight	238.3
Formula	C₉H₁₈O₅S
Cas No.	367-93-1
Smiles	S(C(C)C)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
Relative Density.	1.37 g/cm3

Storage & Solubility Information

Storage

Store under nitrogen Pure form: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 237 mg/mL (994.54 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (20.98 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.1964 mL	20.9820 mL	41.9639 mL	209.8196 mL
5 mM	0.8393 mL	4.1964 mL	8.3928 mL	41.9639 mL
10 mM	0.4196 mL	2.0982 mL	4.1964 mL	20.9820 mL
20 mM	0.2098 mL	1.0491 mL	2.0982 mL	10.4910 mL
50 mM	0.0839 mL	0.4196 mL	0.8393 mL	4.1964 mL
100 mM	0.0420 mL	0.2098 mL	0.4196 mL	2.0982 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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