IPTG
Cat# M6834-5g
Size : 5g
Marca : AbMole Bioscience
Synonym:
Isopropyl β-D-thiogalactoside
Quality Control & Documentation
Biological Activity
IPTG is a used in combination with X-GAL in cloning procedures requiring expression of β-galactosidase activity. Non-metabolizable analog of galactose. This product has <0.1% dioxane content.
Chemical Information
Molecular Weight | 238.3 |
Formula | C9H18O5S |
CAS Number | 367-93-1 |
Solubility (25°C) | Water ≥ 70 mg/mL DMSO 50 mg/mL |
Storage | Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Conversion of different model animals based on BSA (PMID: 27057123)
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
References
[1] Calloe, et al. Cardiovasc Res. A transient outward potassium current activator recapitulates the electrocardiographic manifestations of Brugada syndrome.