KLN-205 genomic DNA - 5 microgram
Marca : CLS Cell Lines Service
KLN-205 Cells
General information
Description | KLN 205 cells form metastatic lesions in lungs after inoculation into mice. Tested and found negative for ectromelia virus (mousepox). |
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Organism | Mouse |
Tissue | Lung |
Disease | Squamous cell carcinoma |
Synonyms | KLN 205, KLN205 |
Characteristics
Growth properties | Adherent |
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Identifiers / Biosafety / Citation
Citation | KLN-205 (Cytion catalog number 400419) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in DBA/2 and BDF1 mice |
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Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypLE Express (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 degree Celsius for 10 -15 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
Split ratio | A ratio of 1:5 is recommended |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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