LEctPROFILE® Affinity Gel VFA

LEctPROFILE® gels are affinity gel chromatography where lectins are immobilized on a Sepharose 4B fast flow matrix. Glycoconjugates can be recovered by competition with the specific inhibitory monosaccharide of the lectin. LEctPROFILE gels are used for the purification of glycoconjugates with specific N-glycan residues. LectPROFILE gel are personnalized reagents produced on your request (see the list of available naturals and recombinants lectins) in 1, 2 or 5 mL. The binding capacity on LEctPROFILE gel of glycoprotein is over 1 mg per mL of gel.

Applications

Purification of polysaccharides.
Purification of complex glycoproteins having specific glycans structures.

Preparation and quality control

Home made lectins affinity chromatography media (LEcPROFILE® gel) prepared in our laboratory.

Useful for purification of glycoconjugates (i.e. separation of various family of glycoconjugates, extraction from natural sources or purification of recombinant therapeutic proteins).

Each LEctPROFILE® gel is carefully controled according to our specifications:

• Quantity of lectin/mL of media
• Capacity of the product (assessed with reference glycoproteins)
• LEctPROFILE® gel are available in 1, 2 or 5 mL slurry. Pre-packed column are also available upon request (sup. Option).