Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Liproxstatin-1 [950455-15-9]

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Cat# NB-64-13035-5mg

Size : 5mg

Marca : Neo Biotech

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Liproxstatin-1

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Catalog No. T2376 Copy Product Info

Purity: 99.72%

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Liproxstatin-1 is a potent and selective inhibitor of ferroptosis (IC50=22 nM). Liproxstatin-1 protects cells from ferroptosis induced by ferroptosis inducers (e.g., Erastin, RSL3).

Cas No. 950455-15-9

With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.72%

Product Introduction

Bioactivity

Description	Liproxstatin-1 is a potent and selective inhibitor of ferroptosis (IC50=22 nM). Liproxstatin-1 protects cells from ferroptosis induced by ferroptosis inducers (e.g., Erastin, RSL3).
Targets & IC50	Fibroblast:22 nM (EC50), Lymphocyte:22 nM (EC50), Ferroptosis:22 nM
In vitro	METHODS: Mouse fibroblasts L929 were treated with Liproxstatin-1 (0-250 nM) for 24 h. Cell viability was measured using AquaBluer. RESULTS: Liproxstatin-1 protected against FINs such as BSO (10 μM), erastin (1 μM), and RSL3 (0.5 μM) in a dose-dependent manner, while it did not rescue staurosporine (0.2 μM) or H2O2 (200 μM)-induced cell death. [1] METHODS: Oligodendrocytes OLN93 were treated with Liproxstatin-1 (1 μM) and RSL-3 (7.89 μM) for 24 h, and GSH levels were measured by micro reduced GSH assay kit. RESULTS: Liproxstatin-1 treatment increased GSH levels compared to the RSL-3 group. [2]
In vivo	METHODS: To test the potential to prevent animal-induced Gpx4 destruction in vivo, Liproxstatin-1 (10 mg/kg) was administered intraperitoneally to TAM-treated CreERT2;Gpx4fl/fl mice once daily for two weeks. RESULTS: Liproxstatin-1 significantly prolonged survival. the number of TUNEL+ cells in the Liproxstatin-1 group was significantly reduced, and the table Liproxstatin-1 delayed the desmoplasia of renal tubular cells. [1] METHODS: To investigate the role of ferroptosis in inflammation-associated cognitive deficits, Liproxstatin-1 (10 mg/kg) was administered intraperitoneally to C57BL/6 mice once daily for five days followed by LPS administration. RESULTS: Liproxstatin-1 ameliorated memory deficits in a mouse model of LPS-induced cognitive impairment.The protective effects of Liproxstatin-1 were associated with attenuation of iron deposition and modulation of the iron-death-associated protein families, TF, xCT, Fth, Gpx4 and FtMt. [3]
Cell Research	Cell viability is assessed at different time points after treatment using AquaBluer according to the manufacturer’s recommendations. Alternatively, cell death is also quantified by measuring released lactate dehydrogenase activity using the cytotoxicity detection kit (LDH). (Only for Reference)

Chemical Properties

Molecular Weight	340.85
Formula	C₁₉H₂₁ClN₄
Cas No.	950455-15-9
Smiles	Clc1cccc(CNC2=Nc3ccccc3NC22CCNCC2)c1
Relative Density.	1.32 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: soluble

H2O: Insoluble

DMSO: 255 mg/mL (748.13 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 6 mg/mL (17.6 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9338 mL	14.6692 mL	29.3384 mL	146.6921 mL
5 mM	0.5868 mL	2.9338 mL	5.8677 mL	29.3384 mL
10 mM	0.2934 mL	1.4669 mL	2.9338 mL	14.6692 mL
20 mM	0.1467 mL	0.7335 mL	1.4669 mL	7.3346 mL
50 mM	0.0587 mL	0.2934 mL	0.5868 mL	2.9338 mL
100 mM	0.0293 mL	0.1467 mL	0.2934 mL	1.4669 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Keywords

Liproxstatin-1Liproxstatin1Liproxstatin 1InhibitorinhibitFerroptosis

Related Tags: Liproxstatin-1 chemical structure | Liproxstatin-1 in vivo | Liproxstatin-1 in vitro | Liproxstatin-1 formula | Liproxstatin-1 molecular weight