Live-or-Dye™ 568/583 Fixable Viability Staining Kit, Trial Size (50 assays)

Cat# 32005-T

Size : 1kit

Marca : Biotium

Contatta il distributore locale :


Telefono : +1 850 650 7790

Live-or-Dye™ Fixable Viability Staining Kits

Kits to covalently label dead cells, allowing cells with permeable plasma membranes to be excluded from analysis in flow cytometry. A wide variety of dye options for standard flow, spectral flow, and fluorescence microscopy.

Technology

Cell Viability & Apoptosis
Flow Cytometry Reagents
Live-or-Dye™ Fixable Viability Stains

Product Description

Live-or-Dye™ Fixable Viability Staining Kits allow discrimination between live and dead cells by flow cytometry or microscopy. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.

Features

  • Highly dead-cell specific
  • Covalent labeling with reactive dyes
  • Withstand fixation and permeabilization
  • Wide selection of colors
  • Dye options designed for spectral flow
  • Sampler kits for convenience

Single Color Kit Components

  • Live-or-Dye™ fixable dead cell dye
  • Anhydrous DMSO

Sampler Kit Components

  • 5 different Live-or-Dye™ fixable dead cell dyes
  • Anhydrous DMSO

Biotium offers a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis. Visit the Live-or-Dye™ Technology Page for more data and information.

How It Works

Live-or-Dye™ Fixable Viability Stains are cell membrane impermeant amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.

Biotium has a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum (see Table 1 below), for maximal flexibility in designing multi-color flow panels.

We also offer two sampler kits, each containing five different dye colors, for excitation from all of the popular flow cytometry laser lines (see Table 2 below). The Live-or-Dye™ Sampler Kit, Standard (32016) was designed for use on the most common flow cytometer laser and filter configurations, with dyes excitable by UV, Violet, Blue, Yellow-Green, and Red lasers. The Live-or-Dye™ Sampler Kit, Spectral (32017) was designed for use in spectral scanning flow cytometry. It contains dyes excitable by UV, Violet, Blue, and Red lasers, all of which have been validated on the Cytek® Aurora spectral cytometer, and chosen for their ability to fill the gaps in many spectral flow panels.

Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Table 1: Single-Color Live-or-Dye™ Kits

Cat. No.

Viability Dye

Compatible lasers (nm)

Optimal detection channels

Notes

32018, 32018-T Live-or-Dye™ 330/410 355, 375 BUV395
32002, 32002-T Live-or-Dye™ 350/448 355, 375 DAPI
32014,32014-T Live-or-Dye™ 375/600 355, 375, 405 Spectral scan, BUV615, BV605 Developed for spectral cytometry
32003, 32003-T Live-or-Dye™ 405/452 405 Pacific Blue®, BV421, BV450
32009, 32009-T Live-or-Dye™ 405/545 405 AmCyan, BV510
32004, 32004-T Live-or-Dye™ 488/515 488 FITC Validated for microscopy
32012, 32012-T Live-or-Dye™ 510/550 488, 532 Spectral scan Developed for spectral cytometry
32005, 32005-T Live-or-Dye™ 568/583 488, 532, 561 PE, PI Validated for microscopy
32006, 32006-T Live-or-Dye™ 594/614 488, 532, 561 PI, PE-CF®594, PE-Texas Red® Validated for microscopy
32015, 32015-T Live-or-Dye™ 615/740 633-640 Spectral scan, APC-Cy®7 Developed for spectral cytometry
32007, 32007-T Live-or-Dye™ 640/662 633-640 APC Validated for microscopy
32013, 32013-T Live-or-Dye™ 665/685 633-640 Spectral scan, AF700 Developed for spectral cytometry
32008, 32008-T Live-or-Dye™ 750/777 633-640, 785 APC-Cy®7 The brightest dye for the APC-Cy®7 channel
32011, 32011-T Live-or-Dye™ 787/808 785, 808 APC-Cy®7, IR800

Table 2: Live-or-Dye™ Sampler Kits

Kit Name

Catalog No.

Included dyes: Ex/Em (Cat No.)

Application

Live-or-Dye™ Fixable Viability Sampler Kit, Standard 32016 • 350/448 (32002A)
• 405/545 (32009A)
• 488/515 (32004A)
• 568/583 (32005A)
• 640/662 (32007A)
Designed for use on most standard flow cytometry laser and filter configurations
Live-or-Dye™ Fixable Viability Sampler Kit, Spectral 32017 • 350/448 (32002A)
• 375/600 (32014A)
• 510/550 (32012A)
• 615/740 (32015A)
• 665/685 (32013A)
Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores
 

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