Disease:

Breast adenocarcinoma

Origin:

Isolated from pleural effusions of a breast cancer patient. Cells exhibit some features of differentiated mammary epithelium including estradiol synthesis and formation of domes. Cells express both the wild type and variant estrogen receptors as well as progesterone receptor.

Species:

Homo sapiens

Tissue:

Breast, mammary gland

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

2n = 46, hypertriploid to hypotetraploid.

Patient:

Female, Caucasian, 69 yrs of age

Medium:

AddexBio-formulated EMEM (C0005-01) + 0.01 mg/ml human recombinant insulin + 10% Fetal Bovine Serum (FBS).

Subculture:

1:2 to 1:6 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I

Sterility: