TP315661 | MDA5 (IFIH1) (NM_022168) Human Recombinant Protein Clinisciences

MDA5 (IFIH1) (NM_022168) Human Recombinant Protein

MVPro

Full-length human proteins expressed in HEK293T cells

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SKU

TP315661

Recombinant protein of human interferon induced with helicase C domain 1 (IFIH1), 20 µg

Comparison of each of the chemokine levels (CCL2, CXCL11, CXCL9, CCL17, CXCL10) between anti-MDA5 antibody-positive and -negative patients. Anti-MDA5 antibody status was determined by ELISA using recombinant MDA5/IFIH1 antigen (OriGene TP315661). The Mann-Whitney U-test estimated the P value. * P < 0.05. Figure cited from Sci Rep, PMID: 28487565

Specifications
Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence >RC215661 representing NM_022168 Red=Cloning site Green=Tags(s) MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGV WHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCME EELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSESNAEIE NLSQVDGPQVEEQLLSTTVQPNLEKEVWGMENNSSESSFADSSVVSESDTSLAEGSVSCLDESLGHNSNM GSDSGTMGSDSDEENVAARASPEPELQLRPYQMEVAQPALEGKNIIICLPTGSGKTRVAVYIAKDHLDKK KKASEPGKVIVLVNKVLLVEQLFRKEFQPFLKKWYRVIGLSGDTQLKISFPEVVKSCDIIISTAQILENS LLNLENGEDAGVQLSDFSLIIIDECHHTNKEAVYNNIMRHYLMQKLKNNRLKKENKPVIPLPQILGLTAS PGVGGATKQAKAEEHILKLCANLDAFTIKTVKENLDQLKNQIQEPCKKFAIADATREDPFKEKLLEIMTR IQTYCQMSPMSDFGTQPYEQWAIQMEKKAAKEGNRKERVCAEHLRKYNEALQINDTIRMIDAYTHLETFY NEEKDKKFAVIEDDSDEGGDDEYCDGDEDEDDLKKPLKLDETDRFLMTLFFENNKMLKRLAENPEYENEK LTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNE QKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGV IERETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNLSLITFLCKNC SVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKTLQKKCADYQINGEIICKCGQAWGTMMVHKGLDLP CLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Predicted MW	116.5 kDa
Concentration	>0.1 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity	ELISA capture for autoantibodies (PMID: 28487565) ELISA capture for autoantibodies (PMID: 28842784)
Preparation	Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping	Dry Ice
Reference Data
RefSeq	NP_071451
Locus ID	64135
UniProt ID	Q9BYX4
Cytogenetics	2q24.2
RefSeq Size	3434
RefSeq ORF	3075
Synonyms	AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2; SGMRT1
Summary	IFIH1 encodes MDA5 which is an intracellular sensor of viral RNA that triggers the innate immune response. Sensing RNA length and secondary structure, MDA5 binds dsRNA oligonucleotides with a modified DExD/H-box helicase core and a C-terminal domain, thus leading to a proinflammatory response that includes interferons. It has been shown that Coronaviruses (CoVs) as well as various other virus families, are capable of evading the MDA5-dependent interferon response, thus impeding the activation of the innate immune response to infection. MDA5 has also been shown to play an important role in enhancing natural killer cell function in malaria infection. In addition to its protective role in antiviral responses, MDA5 has been implicated in autoimmune and autoinflammatory diseases such as type 1 diabetes, systemic lupus erythematosus, and Aicardi-Goutieres syndromeprovided by RefSeq, Jul 2020
Protein Categories	Cytokines, Diabetes, Immune system diseases, Intracellular Proteins
Protein Pathways	RIG-I-like receptor signaling pathway

FAQs
Protein FAQs
SDS
SDS for TP315661

Recombinant Protein Resources
Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

SKU	Description	Size
PH315661	IFIH1 MS Standard C13 and N15-labeled recombinant protein (NP_071451)	10 ug
LC411723	Lyophilized IFIH1 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LY411723	Transient overexpression lysate of interferon induced with helicase C domain 1 (IFIH1) (as WB positive control)	100 ug

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

MDA5 (IFIH1) (NM_022168) Human Recombinant Protein

Cat# TP315661

MDA5 (IFIH1) (NM_022168) Human Recombinant Protein

Citations

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MDA5 (IFIH1) (NM_022168) Human Recombinant Protein

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Citations