Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse Anti-human IgE secondary antibody, clone 4F7 (Cat# MAB12445) under 2 ug/mL working concentration.
- Specifications
Product Description
Mouse monoclonal antibody raised against human IgE.
Immunogen
Human IgE whole molecule.
Host
Mouse
Reactivity
Human
Specificity
This product is an IgG2a kappa antibody that was purified from tissue culture. It reacts with human IgE by indirect ELISA and does not react with IgM, IgA or IgG. > 90% pure as determined by page gel.
Form
Lyophilized
Purification
Antigen Affinity Purified
Isotype
IgG2a
Recommend Usage
ELISA (2 ug/mL)
Immunoprecipitation (2 ug/mg)
Western Blot (0.05-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.2
Storage Instruction
Store at 4°C.
After reconstitution with 1 mL of reagent quality water, aliquots should be stored at -20°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse Anti-human IgE secondary antibody, clone 4F7 (Cat# MAB12445) under 2 ug/mL working concentration.Immunoprecipitation
Western Blot