Mouse IgG1 Antibody : CY5
Cat# OASB00020
Size : 100tests
Marca : Aviva Systems Biology
Mouse IgG1 Antibody : CY5 (OASB00020)
| Datasheets/Manuals | Printable datasheet for Mouse IgG1 Antibody : CY5 (OASB00020) |
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| Clonality | Monoclonal |
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| Clone | 15H6 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | Cyanine 5 |
| Application | FC, FLISA, IHC-F, IHC-P, ICC, WB |
| Reconstitution and Storage | Store at 2-8C |
| Concentration | Lot specific |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: ELISA: Purified antibody Fluorescein conjugate Biotin conjugate PE and APC conjugates PE-TXRD conjugate Spectral Red conjugate Cyanine 5 conjugate PE-CY 5.5 and PE-CY7 conjugates APC-CY 5.5 and APC-CY7 conjugates AF488 and AF647 conjugates Purified antibody AP conjugate HRP conjugate <=1 ug/106 cells 10 uL/106 cells <=1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 1-5ug/mL 1:2,000-1:4,000 1:2,000-1:4,000 |
| Application Info | Flow cytometry, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA) |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), Cyanine 5 (CY 5), Alexa Fluor 488 (AF488), and Alexa Fluor 647 (AF647)conjugates are supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin- Texas Red (PE- TXRD), R- phycoerythrin- Cyanine 5.5 (PECY5.5), R- phycoerythrin- Cyanine 7 (PE- CY 7), Allophycocyanin- Cyanine 5.5 (APC- CY 5.5) andAllophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed. |
| Gene Full Name | Ig gamma-1 chain C region |
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