Mouse IgG3 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommended Dilution	WB: 1:500 - 1:2,000 IHC: 1:200 - 1:1,000 ELISA: 1:2,000 - 1:10,000
Reactivities	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Mouse IgG3 heavy chain
Specificity	Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Alkaline Phosphatase
Storage	Store vial at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Note	Anti-Mouse IgG3 Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Alkaline Phosphatase Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types.