Mouse Monoclonal Anti-Insulin-like Growth Factor-1 (IGF-1) [Clone IGF1/1020]
Cat# NB-36-00319-P1
Size : 100ug
Marca : Neo Biotech
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Insulin-like Growth Factor-1 (IGF-1) Antibody [IGF1/1020]
Immunofluorescence Analysis of HeLa cells labeling IGF-1. IGF-1 Mouse Monoclonal Antibody (IGF/1020) conjugated to CF647R (green). DAPI (blue) nuclear counterstain.
Applications & Dilutions
Summary
This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.
Product Properties & Targets
Functions
- The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23696648, PubMed:23243309).
Key References
- Rotwein p, et. al. (1986) J. Biol. Chem. 261: 4828-4832.
- Sandberg-Nordqvist AC, et. al. (1993) Cancer Res. 53: 2475-2478.
- Zheng WH, et. al. (2000) J. Neural.Transm. Suppl. 2000: 261-272.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/6358902/
- https://pubmed.ncbi.nlm.nih.gov/3002851/
- https://pubmed.ncbi.nlm.nih.gov/2935423/
- https://pubmed.ncbi.nlm.nih.gov/2937782/
- https://pubmed.ncbi.nlm.nih.gov/3455760/
- https://pubmed.ncbi.nlm.nih.gov/2082190/
- https://pubmed.ncbi.nlm.nih.gov/2018498/
- https://pubmed.ncbi.nlm.nih.gov/1372070/
- https://pubmed.ncbi.nlm.nih.gov/8495408/
- https://pubmed.ncbi.nlm.nih.gov/16541075/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/6382022/
- https://pubmed.ncbi.nlm.nih.gov/632300/
- https://pubmed.ncbi.nlm.nih.gov/3242681/
- https://pubmed.ncbi.nlm.nih.gov/19578119/
- https://pubmed.ncbi.nlm.nih.gov/20881139/
- https://pubmed.ncbi.nlm.nih.gov/21076856/
- https://pubmed.ncbi.nlm.nih.gov/22351760/
- https://pubmed.ncbi.nlm.nih.gov/23243309/
- https://pubmed.ncbi.nlm.nih.gov/23696648/
- https://pubmed.ncbi.nlm.nih.gov/24132240/
- https://pubmed.ncbi.nlm.nih.gov/6189745/
- https://pubmed.ncbi.nlm.nih.gov/2036417/
- https://pubmed.ncbi.nlm.nih.gov/1319992/
- https://pubmed.ncbi.nlm.nih.gov/8857020/
- https://pubmed.ncbi.nlm.nih.gov/10391209/
- https://pubmed.ncbi.nlm.nih.gov/24389050/
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