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Mouse Monoclonal Anti-MUC3 (Mucin 3) [Clone MUC3/1154]
Cat# NB-36-00530-P0
Size : 20ug
Marca : Neo Biotech
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Telefono : +1 850 650 7790
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Printable Datasheet
MUC3 (Mucin 3) Antibody [MUC3/1154]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).
Applications & Dilutions
Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, Infectious Disease
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Membrane, Secreted
Gene Name
MUC3A
Positive Control
LS174T cells. Human small intestine (IHC).
Immunogen
A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
Alternate Names
Mucin-3A, Intestinal mucin-3A, Intestinal mucin-3A; intestinal mucin-like; MUC3; MUC3A; MUC3B; mucin 3, intestinal; mucin 3A, cell surface associated; mucin 3A, intestinal; mucin 3B, cell surface associated
Functions
- Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. May be involved in ligand binding and intracellular signaling.
Key References
- Gum, J.R. Jr., et al. 1990. Molecular cloning of cDNAs derived from a novel human intestinal Mucin gene. Biochem. Biophys. Res. Commun 171: 407-415.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/12853948/
- https://pubmed.ncbi.nlm.nih.gov/12958310/
- https://pubmed.ncbi.nlm.nih.gov/9334251/
- https://pubmed.ncbi.nlm.nih.gov/10512748/
- https://pubmed.ncbi.nlm.nih.gov/11289722/
- https://pubmed.ncbi.nlm.nih.gov/10405327/
- https://pubmed.ncbi.nlm.nih.gov/2393399/
- https://pubmed.ncbi.nlm.nih.gov/18669648/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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