Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Mucicarmine [51395-97-2]

Mucicarmine [51395-97-2]

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Cat# HY-D1542-5g

Size : 5g

Marca : MedChemExpress


Description

Mucicarmine is used in the histological visualization of acid mucopolysaccharides in tissue sections. Mucicarmine can identify mucin (deep rose), which is useful in determining the mucin positive cancer (such as liver carcinoma)[1].

IC50 & Target

Mucin[1]
In Vitro

Mucicarmine successfully stains the presence of mucin on frozen-tissue sections (Mucin stained deep rose).
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Mount the cryostat sections (thickness: 4 to 5 μm) onto coated glass slides.
2. Fix the sections in frozen-section fixative immediately for 30 seconds and then rinse with water for 5 seconds.
3. Immerse the glass slides for 30 seconds into Harris hematoxylin, then consecutively into distilled water, ammonium hydroxide, and distilled water, for 5 seconds in each solution.
4. The mucicarmine stain is placed in the microwave oven and microwave until the solution is about to bubble (30 seconds).
5. Immerse the glass slides in the warmed mucicarmine solution for 2 minutes and rinse slides in distilled water for 5 seconds.
7. Immerse the glass slides consecutively in 95% alcohol, 100% alcohol, and xylene for 5 seconds in each solution, then coverslip the slides.
Note: Nuclei stain (blue) and connective tissue stain (green) maybe is necessary according to your specific needs.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mucicarmine Related Antibodies

Solvant et solubilité
In Vitro: 

H2O : 25 mg/mL (Need ultrasonic)

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
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