NeoLume™ ECL Substrate – Ultra-Femto
Description
NeoLume is a line of chemiluminescent substrates for horseradish peroxidase (HRP)-based immunoassays, dedicated to manufacturers of chemiluminescent immunoassay (CLIA) kits.
The line comprises six substrates in a two-solution format, covering sensitivity levels from the mid-picogram to the attogram range. These easy-to-use substrates combine sensitivity, specificity, signal stability, and lot-to-lot consistency.
Chemiluminescent substrates are particularly suited to quantitative immunoassays requiring an extended dynamic range and qualitative assays requiring very low detection limits. The bright signal can be measured within one minute, avoiding the long incubations or addition of stopping reagents associated with traditional colorimetric assays.
NeoLume substrates are available in kit format and bulk. Proprietary technology enables fine-tuning of signal intensity, and custom substrates can be developed to meet assay manufacturers’ sensitivity requirements.
Key Benefits
- Different sensitivity levels: six substrates spanning mid-picogram to attogram detection.
- High lot-to-lot consistency: negligible variation minimizes assay re-optimization and supports reproducibility.
- Stable working solutions: at least 3 days at room temperature.
- Room-temperature storage.
- Excellent signal stability: selected substrates provide signal duration of up to 20 hours.
- Flexible supply: kit and bulk formats, with custom substrate development available.
Signal Stability and Reproducibility
All NeoLume substrates are formulated to ensure negligible lot-to-lot variation, minimizing assay re-optimization and supporting reproducible performance.
Working solutions remain stable at room temperature for at least 3 days. NeoLume Very High Sensitivity and NeoLume Ultra Sensitivity provide an extended signal duration of up to 20 hours.
NeoLume Attogram Sensitivity
NeoLume Attogram Sensitivity is the most sensitive option in the NeoLume family. Its specific proprietary technology combines extreme sensitivity with exceptionally high light output, enabling detection of trace amounts of proteins at the attogram level.
The formulation provides negligible background and an extremely high signal-to-noise ratio.
Product Properties
| Property | Details |
|---|---|
| Product line | NeoLume chemiluminescent substrates. |
| Application | Chemiluminescent immunoassays (CLIA). |
| Enzyme | Horseradish peroxidase (HRP). |
| Intended users | CLIA kit manufacturers. |
| Assay suitability | Quantitative assays requiring an extended dynamic range and qualitative assays requiring very low detection limits. |
| Format | Six substrates in a two-solution format. |
| Sensitivity range | Mid-picogram to attogram detection. |
| Signal measurement | Within 1 minute. |
| Lot-to-lot consistency | High consistency with negligible variation, minimizing assay re-optimization. |
| Working solution stability | At least 3 days at room temperature. |
| Extended signal duration | Up to 20 hours with NeoLume Very High Sensitivity and NeoLume Ultra Sensitivity. |
| Highest-sensitivity option | NeoLume Attogram Sensitivity: attogram-level protein detection, negligible background, and an extremely high signal-to-noise ratio. |
| Storage | Room temperature. |
| Supply | Kit format and bulk. |
| Customization | Fine-tuning of signal intensity and custom substrates based on assay manufacturers’ sensitivity requirements. |