NAO is a green fluorescent mitochondrial dye whose staining is not dependent on mitochondrial membrane potential (1), unlike JC-1 (70011) or Rhodamine 123 (70010). NAO has been used to study multidrug resistance (2) and to measure changes in mitochondrial mass during apoptosis (3). The dye is reported to bind cardiolipin, a phosphoprotein in the inner mitochondrial membrane (4). Also see our MitoView™ Dyes and other mitochondrial dyes.

• λ_Ex/λ_Em (MeOH) = 495/522 nm
• ε (MeOH) = 63,000
• Orange solid soluble in DMSO or DMF
• Store at 4°C and protect from light, especially in solution
• C₂₆H₃₈BrN₃
• MW: 473
• [75168-11-5]