Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1)

Cat# MBS653730-100U

Size : 100Units

Marca : MyBiosource


Nuclease P1 Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653730

MBS653730 is an enzyme targeting Nuclease P1. Also known as Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1). Host: Penicillium citrinum. More details

Product Overview

Online Datasheet in PDF
Product Name
Nuclease P1, Enzyme
Full Product Name
Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1)
Host
Penicillium citrinum
Form/Format
Supplied as a powder lyophilized from a solution containing 30mM sodium acetate buffer, pH 5.3 with 5mM ZnCl2 and 50mM sodium chloride. Enzyme suspension obtained after reconstitution.
Purity/Purification
Molecular Biology Grade
Concentration
Protein Concentration (before lyophilization): 1.50 mg/ml (lot specific)
Specificity
410u/mg protein
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 12 months. Reconstituted product should be aliquoted and stored at -20°C. Reconstituted product is stable for 2-3 weeks at 4°C and 3-6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
42-50kD
Specific Activity
521 u/mg protein
Stable pH
5.0-8.0
Optimum pH
5.3 for RNA and heat-denatured DNA
7.2 for 3' -AMP
Optimum Temperature
~70°C [<60°C for long incubations]
Reconstitution
For maximum recovery of product, briefly centrifuge the original vial prior to opening. This will dislodge any enzyme that has adhered to the side or in the cap due to shipping. Reconstitute with sterile ddH2O. Allow the nuclease to rest on ice for ~10-15 minutes. Vortex gently to ensure there is uniform suspension (Nuclease tends to become layered towards the bottom of the vial). Just before addition to the reaction mixture, gently vortex the suspension.
Unit Definition
One unit of nuclease activity is defined as the amount of enzyme that produces 1umole of acid soluble nucleotides from RNA based on E = 10,600 for RNA hydrolysates per minute at 37 degree C, pH 5.3. One unit of the enzyme liberates 1umole of orthophosphate from 3'-AMP per minute at 37 degree C, pH 7.2
Substrate specificity
The enzyme hydolyzes both 3'-5' phosphodiester bonds in RNA and heat-denatured DNA, and 3'-phosphomonoester bonds in mono and oligonucleotides terminated with a 3'-phosphate group without base specificity. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0.

Product Categories/Family for Nuclease P1 enzyme

Molecular Biology; MB-Enzymes

Testing Data of Nuclease P1 enzyme

MBS653730 Nuclease P1 100U reconstituted with 100ul sterile, ddH2O. Let rest 30 minutes and vortex. Vortex before every use.
MBS657782 RNA, Torula Yeast 10mg/1ml 0.1M sodium acetate.
Lanes 1, 2,7 and 8 are MBS657782 RNA, Torula Yeast.
Lanes 3 and 4 are MBS657782 RNA, Torula Yeast and MBS653730 Nuclease P1 C5012050.
Lanes 5 and 6 are MBS657782 RNA, Torula Yeast and MBS653730 Nuclease P1 C5120101
Enzyme – Cat# MBS653730

Testing Data of Nuclease P1 enzyme

Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1)
Enzyme, Testing Data – Cat# MBS653730

Testing Data of Nuclease P1 enzyme

Gen. Ref. 6:
Autoradiogram of 32P-postlabeled nuclease P1-treated DNA from photo-irradiation in the presence of (A) DMBA, (B) blank, (C) 7-HOCH2-12-MBA, (D) 12-HOCH2-7-MBA, (E) 7-CHO-12-MBA, and (F) 12-CHO-7-MBA in THF and calf thymus DNA by UVA light at a total dose of 14 J/cm2.
Enzyme, Testing Data – Cat# MBS653730

Testing Data of Nuclease P1 enzyme

EC=3.1.30.1; Endonuclease P1; Nuclease 5'-Nucleotidehydrolase; 3'-Phosphohydrolase; NP1
Enzyme, Testing Data – Cat# MBS653730

Other Notes

Small volumes of Nuclease P1 enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Nuclease P1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
nuclease P1 {EC 3.1.30.1}
Protein Family
Nuclease

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

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