PGI1 Rabbit Polyclonal Antibody

CAT#: AP21370BT-N

PGI1 rabbit polyclonal antibody, Biotin


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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution Can be used for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
Recommended working dilutions: vary widely, but may be between 1/1000 and 1/5000.
Reactivities Bakers Yeast
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Phosphoglucose isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phosphoglucose isomerase from baker’s yeast.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: Biotin
State: Lyophilized hyperimmune IgG fraction
Label: Marker: N-Hydroxysuccinimidobiotin. Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilised to assure stability and long shelf life
Molar radio: Biotin/IgG ~5.0
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Biotin
Storage Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Synonyms Phosphoglucose isomerase, PGI1, Phosphohexose isomerase, PHI, Neuroleukin, NLK
Reference Data

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