Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Pig interleukin 8,IL-8 ELISA Kit

Pig interleukin 8,IL-8 ELISA Kit

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Cat# CSB-E06787p-96T

Size : 96T

Marca : Cusabio

SDS

CSB-E06787p
Size 96T,5×96T,10×96T

Product Details

Target Name
interleukin 8
Associated names
CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; Alveolar macrophage chemotactic factor I ELISA Kit; AMCF-I ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
Abbreviation
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
125 pg/mL-8000 pg/mL
Sensitivity
31.25 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %95
Range %91-99
1:2Average %90
Range %86-94
1:4Average %88
Range %84-93
1:8Average %92
Range %88-97
Recovery
The recovery of pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9995-104
EDTA plasma (n=4)8581-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
80002.662 2.601 2.632 2.460
40002.215 2.161 2.188 2.016
20001.498 1.404 1.451 1.279
10000.871 0.819 0.845 0.673
5000.510 0.507 0.509 0.337
2500.380 0.359 0.370 0.198
1250.271 0.261 0.266 0.094
00.174 0.170 0.172 　
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Pig IL8 ELISA Kit was designed for the quantitative measurement of Pig IL8 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

Citations

  • X Ji, H Ding, F Zhou, F Zhang, D Wu,Ecotoxicology and Environmental Safety,2025
  • Jingxia Gao,Frontiers in immunology,2022
  • F Shi,BMC Veterinary Research,2020
  • Qingwei Meng.et al,J Anim Sci Biotechnol.,2018
  • Vigors S. et al,PLoS One.,2016

Customer Reviews and Q&A

Average Rating:
5.0 - 1 reviews

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