PRDX6 ELISA Kit (Human)
Cat# OKEH01255
Size : 96Wells
Marca : Aviva Systems Biology
Contatta il distributore locale :
Telefono : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology PRDX6 ELISA Kit (Human) (OKEH01255) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRDX6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRDX6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRDX6 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 15.6-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.7% (n = 20) Mean Inter-assay CV%: < 7.5% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 4.6 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Peroxiredoxin-6 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | PRDX6 |
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| Gene Full Name | peroxiredoxin 6 |
| Alias Symbols | 1-Cys;1-Cys peroxiredoxin;1-Cys PRX;24 kDa protein;acidic calcium-independent phospholipase A2;aiPLA2;antioxidant protein 2;AOP2;epididymis secretory sperm binding protein Li 128m;glutathione-dependent peroxiredoxin;HEL-S-128m;liver 2D page spot 40;LPC acyltransferase 5;LPCAT-5;lyso-PC acyltransferase 5;lysophosphatidylcholine acyltransferase 5;non-selenium glutathione peroxidase;NSGPx;p29;peroxiredoxin-6;PRX;red blood cells page spot 12. |
| NCBI Gene Id | 9588 |
| Protein Name | P30041 |
| Description of Target | Peroxiredoxin-6 |
| Uniprot ID | https://www.uniprot.org/uniprot/P30041 |
| Protein Accession # | NP_004896.1 |
| Nucleotide Accession # | NM_004905.2 |
| Protein Size (# AA) | 224 |
| Molecular Weight | 25 kDa |