A highly stable recombinant serine protease from Tritirachium album, ideal for protein digestion and nucleic acid purification in molecular biology. Offers broad substrate specificity and resistance to denaturing agents.
Proteinase K (liquid)
Proteinase K (liquid)
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Product Description
Specifications
| Size | 1 mL, 5 mL |
| Molecular Weight (kDa) | 29 kDa by SDS-PAGE |
| Format | Liquid |
| Activity | ≥800 U/ml |
| Shipping Type | Blue ice |
| Storage | 2-8°C |
Introduction
Recombinant Proteinase K (EC 3.4.21.64) from Tritirachium album is a highly stable serine protease with broad substrate specificity. It effectively degrades a wide range of native proteins, even in the presence of detergents. Evidence from crystal and molecular structure studies indicates this enzyme belongs to the subtilisin family with an active site catalytic triad (Asp39-His69-Ser224). The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. Its versatility makes it a widely used enzyme in molecular biology for protein digestion and nucleic acid purification.
Features
- Enhanced stability and enzymatic activity through directed evolution technologies
- Tolerant to guanidine salts, making it suitable for demanding lysis conditions
- Ultra-pure quality: free from enzyme impurities and heavy metal ions; DNA carryover <5pg/mg.
- Large production capacity of 1,500kg
Applications
- Genetic diagnostic kits
- RNA and DNA extraction kits
- Extraction of non-protein components from tissues and degradation of protein impurities (e.g., in DNA vaccine production and heparin preparation)
- Preparation of chromosomal DNA for pulsed-field gel electrophoresis (PFGE)
- Western blotting
- In vitro diagnostic reagents for enzymatic glycosylated albumin detection
Specification
Appearance: Colorless to light brown liquid
Activity: ≥800 U/ml
Protein concentration: ≥20 mg/ml
DNase, RNase, Nickase: None detected
Molecular weight: 29 kDa (SDS-PAGE)
Isoelectric point: 7.81
Properties
Optimum pH: 7.0-12.0
Optimum temperature: 65℃
pH stability: pH 4.5-12.5 (25℃, 16 hrs)
Thermal stability: Below 50℃ (pH 8.0, 30 min)
Storage stability: Over 90% activity for 12 months at 25℃
Activators: SDS, urea
Inhibitors: DFP, PMSF
Unit Definition
One unit (U) is defined as the amount of enzyme required to hydrolyze casein to produce 1 μmol tyrosine per minute at 37℃.
Storage / Stability
Over 90% activity stored at 25℃ for 12 months. Recommended storage at 2-8℃.