Rabbit Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with FITC Antibody

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to rat IgG, heavy and light chains. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immuno- fluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.