Orb780328-48Tests | Rat Angiopoietin Like Protein 4 (ANGPTL4) ELISA

Rat Angiopoietin Like Protein 4 (ANGPTL4) ELISA Kit

SKU: orb780328

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 4(ANGPTL4).

Research Area

Cardiovascular Research; Metabolism Research

Images & Validation

−

Tested Applications	ELISA
Application Notes	standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ANGPTL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ANGPTL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ANGPTL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ANGPTL4 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ANGPTL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ANGPTL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ANGPTL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ANGPTL4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.61 ng/mL
Concentration	100 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

−

ARP4 ELISA Kit, FIAF ELISA Kit, HFARP ELISA Kit, NL2 ELISA Kit, PGAR ELISA Kit, Pp1158 ELISA Kit, Fasting-Induced Adipose Factor ELISA Kit, Hepatic Angiopoietin-Related Protein ELISA Kit, Hepatic Fibrinogen/Angiopoietin-Related Protein ELISA Kit

−

Rat ANGPTL4 (Angiopoietin-related protein 4) ELISA Kit [orb2280301]
Reactivity:
Rat
Dynamic Range:
0.156-10ng/ml
Sensitivity:
0.094ng/ml
Sizes Available: 48 T, 96 T
Rat ANGPTL4 ELISA Kit (Ready to Use) [orb554629]
Reactivity:
Rat
Dynamic Range:
1.56-100 ng/mL
Sensitivity:
0.61 ng/mL
Sizes Available: 48 T, 96 T
Rat ANGPTL4 ELISA Kit [orb437925]
Reactivity:
Rat
Dynamic Range:
1.56-100 ng/mL
Sensitivity:
0.61 ng/mL
Sizes Available: 48 T, 96 T
Rat ANGPTL4 ELISA Kit [orb410100]
Reactivity:
Rat
Dynamic Range:
7.8 pg/mL-500 pg/mL
Sensitivity:
1.95 pg/mL
Sizes Available: 10 x 96 T, 48 T, 96 T, 5 x 96 T, 24 T
Rat Angiopoietin Like Protein 4 (ANGPTL4) ELISA Kit [orb1667901]
Reactivity:
Rat
Sizes Available: 96 T, 48 T