Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Rat Interleukin 3,IL-3 ELISA KIT

Rat Interleukin 3,IL-3 ELISA KIT

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Cat# CSB-E07436r-96T

Size : 96T

Marca : Cusabio

SDS

CSB-E07436r
Size 96T,5×96T,10×96T

Product Details

Target Name
interleukin 3 (colony-stimulating factor, multiple)
Associated names
Il3 ELISA Kit; Il-3Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 100  
Range % 95-113  
1:4 Average % 91  
Range % 85-97  
1:8 Average % 97  
Range % 92-104  
Recovery
The recovery of rat IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-94  
EDTA plasma (n=4) 93 87-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.436 2.276 2.356 2.214  
100 1.975 2.014 1.995 1.853  
50 1.435 1.372 1.404 1.262  
25 0.868 0.913 0.891 0.749  
12.5 0.553 0.519 0.536 0.394  
6.25 0.387 0.407 0.397 0.255  
3.12 0.285 0.276 0.281 0.139  
0 0.144 0.140 0.142    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Rat IL3 ELISA Kit was designed for the quantitative measurement of Rat IL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

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Target Background

Function
(From Uniprot)
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Subcellular Location
Secreted.
Protein Families
IL-3 family
Tissue Specificity
Activated T-cells, mast cells, natural killer cells.
Database Links

UniGene: