Rat Interleukin 5,IL-5 ELISA KIT
Cat# CSB-E07435r-96T
Size : 96T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
interleukin 5 (colony-stimulating factor, eosinophil)
Associated names
Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 82-91 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 83-99 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 93-107 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-104 | |||||
Recovery
| The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-94 | ||||
| EDTA plasma (n=4) | 94 | 90-100 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
Interleukin 5 is a cytokine that regulates eosinophil development, activation, and survival. As a member of the type I cytokine family, IL-5 is primarily produced by T helper 2 cells, mast cells, and eosinophils themselves. This glycoprotein acts as a key mediator in allergic inflammatory responses and parasitic infections. It promotes eosinophil differentiation from bone marrow precursors and enhances their recruitment to sites of inflammation. IL-5 signaling is particularly important in asthma, allergic rhinitis, and other eosinophil-associated disorders, making it a significant target for...
Citations
- JS Park, YS Lee, JW Seo, HJ Na,Laboratory Animal Research,2025
- Y Xia, P Bin, Y Zhou, M Zhao, J Zhang, W Zhong,Cell Reports,2025
- JH Oh, SH Baek, HW Seo, SH Lee,Journal of Ginseng Research,2025
- W Liu, Y Zhao, Y He, X Yan, J Yu, Q Song, L Zhang,iScience,2024
- P Arora,Chemico-biological interactions,2022
- M Johnathan,Plos one,2021
- El-Din Y M E,Life Sciences,2019
- Li J, et al,Acta Biomaterialia,2019
- Lan Zhang.et al,Journal of Controlled Release,2018
- Tang X.et al,BMC Complement Altern Med.,2017
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Subcellular Location
Secreted.
Protein Families
IL-5 family
Database Links
STRING:
UniGene: