RH IGF-II
Cat# 100-14-10UG
Size : 10UG
Marca : FUJIFILM
Recombinant Human IGF-II
Insulin-like growth factor II (IGF-II) is an important fetal growth hormone made by theca cells during gestation. IGF-II engages the IGF-I receptor (IGF1R) to mediate embryonic growth. IGF-II also binds the sink IGF-II receptor (IGF2R) leading to IGF-II degradation.
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Single Prominent Band at 7.5 kDa
Figure 1. rh IGF-2 (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 7.5 kDa.
Cell based proliferation assay
Figure 2. Recombinant Human IGF-2 induced proliferation of MCF-7 cells
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | Somatomedin A |
| Accession Number | P01344 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 7.5 kDa (67 aa) |
| AA Sequence | AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | Human breast cancer cell line MCF-7 | ≤ 6 ng/mL; ≥ 1.7 x 105 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.