RH IL-2 (AF)
Cat# 100-12AF-10UG
Size : 10UG
Marca : FUJIFILM
Recombinant Human IL-2
Interleukin 2 (IL-2) is an immunomodulatory cytokine that is produced by lymphocytes. IL-2 signals through the IL-2R receptor to induce activated T cell proliferation and promote T cell differentiation. IL-2 also stimulates the proliferation and differentiation of B cells, natural killer cells, monocytes, and macrophages.
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Single Prominent Band at 15.5 kDa
Figure 1. rh IL-2 (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 15.5 kDa.
Cell based proliferation assay
Figure 2. Recombinant Human IL2 (parental) induced proliferation of CTLL-2 cells
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | T cell growth factor, TCGF, Aldesleukin |
| Accession Number | P60568 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 15.5 kDa (134 aa) |
| AA Sequence | MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Product Reconstitution | Sterile 10 mM HCl at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 97% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | CTLL-2 cell proliferation | ≤ 3 ng/mL; 3.3 x 105 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.