RH IL-3 (AF)
Cat# 100-80AF-100UG
Size : 100UG
Marca : FUJIFILM
Recombinant Human IL-3
Interleukin 3 (IL-3) is a cytokine that is produced by activated T cells and mast cells. IL-3 induces the differentiation of hematopoietic stem cells into myeloid precursor cells, such as erythrocyte, megakaryocyte, granulocyte, monocyte, and dendritic cells. IL-3 also functions in the nervous system and is important during the B-1 cell regulation of chronic inflammatory diseases.
Also available as a GMP product: CTGrade GMP rh IL-3
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Prominent Band at 15.2kDa
Figure 1. rh IL-3 (1 μg) was resolved with SDS-PAGE under non-reducing (NR) conditions and visualized by Instant Blue staining showing a single prominent band at 15.2 kDa.
Cell based proliferation assay
Figure 2. Recombinant human IL3 induced proliferation of TF-1 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | MCGF, Multi-CSF, HCGF, P-cell stimulating factor |
| Accession Number | P08700 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 15.2 kDa (134 aa) |
| AA Sequence | MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 97% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | TF-1 cell proliferation | ≤ 2 ng/mL |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.