RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
Cat# MBS653023-10L
Size : 10L
Marca : MyBiosource
RPMI Culture Media – 1640 Medium W/O Amino Acids, Sodium Phosphate
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS653023RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and… More details
Product Overview
Product Name
RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate, Culture Media
Full Product Name
RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment.
Prepared media should be kept at 4°C and used within a short period of time.
Prepared media should be kept at 4°C and used within a short period of time.
Grade
Cell Culture Grade
Appearance
Light orange, homogenous, free flowing powder
Solubility
Yellow, clear complete
pH
4.9
Endotoxin
< 1 EU/ml
Directions per Liter
Dissolve 8.59g/L in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as mg/liter
Inorganic Salts:
Calcium Nitrate·4H2O: 100
Magnesium Sulfate: 48.84
Potassium Chloride: 400
Sodium Chloride: 6000
Vitamins:
P-Aminobenzoic Acid: 1
D-Biotin: 0.2
Choline Chloride: 3
Cyanocobalamin: 0.005
Folic Acid: 1
myo-Inositol: 35
Niacinamide: 1
D-PantothenicAcid·Ca: 0.25
Pyridoxine·HCl: 1
Riboflavin: 0.2
Thiamine·HCl: 1
Other:
D-Glucose: 2000
Glutathione: 1
Phenol Red, Sodium: 5.3
Total: 8.59g/L
Calcium Nitrate·4H2O: 100
Magnesium Sulfate: 48.84
Potassium Chloride: 400
Sodium Chloride: 6000
Vitamins:
P-Aminobenzoic Acid: 1
D-Biotin: 0.2
Choline Chloride: 3
Cyanocobalamin: 0.005
Folic Acid: 1
myo-Inositol: 35
Niacinamide: 1
D-PantothenicAcid·Ca: 0.25
Pyridoxine·HCl: 1
Riboflavin: 0.2
Thiamine·HCl: 1
Other:
D-Glucose: 2000
Glutathione: 1
Phenol Red, Sodium: 5.3
Total: 8.59g/L
Product Categories/Family for RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate culture media
Other Notes
Small volumes of RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate purchase
MBS653023 is a culture media targeting RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate. Also known as RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder).
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