RPMI 2650 growing culture
Marca : CLS Cell Lines Service
RPMI 2650 Cells
General information
Description | The cells are positive for keratin by immunoperoxidase staining. |
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Organism | Human |
Tissue | Nasal septum |
Disease | Squamous cell carcinoma |
Metastatic site | Pleural effusion |
Synonyms | RPMI-2650, RPMI2650, Roswell Park Memorial Institute 2650 |
Characteristics
Age | 52 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | RPMI 2650 (Cytion catalog number 300323) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, B |
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Reverse transcriptase | Negative |
Products | Mucoid, keratin |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 48 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Fluid renewal | 2 times per week |
Freezing recovery | After thawing allow the cells to recover from the freezing process for at least 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 9,11 D13S317: 11,12 D16S539: 11,12 D5S818: 12,13 D7S820: 8,11 TH01: 6,8 TPOX: 8 vWA: 16,18 D3S1358: 17 D21S11: 28,33.2 D18S51: 16 Penta E: 11,19 Penta D: 9,10 D8S1179: 9,13 FGA: 23,25 |
HLA alleles | A*: 02:01:01, 03:01:01 B*: 07:02:01, 35:01:01 C*: 03:03:01, 07:02:01 DRB1*: 07:01:01, 08:01:01G DQA1*: 02:01:01, 04:01:01 DQB1*: 03:03:02, 04:02:01 DPB1*: 04:01:01 E: 01:01:01, 01:03 |