S6 Ribosomal Protein, phosphorylated (Ser235/236)

To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation. Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions. This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors. The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a 19 amino acid region in the S6 carboxy-terminus.

Suitable for use in Immunofluoresence/Immunocytochemistry and Western Blot. Other applications not tested.

Western Blot: 1:1000. Good for 10 mini blots.
Immunofluorescence (IC): 1:400-800. Fixative: 10% neutral buffered formalin
Optimal dilutions to be determined by the researcher.

For Western blots, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Mab

IgG

5i129(91B2)

rabbit

human

Not Determined

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, <0.02% sodium azide

Purified

Synthetic phospho-peptide corresponding to residues surrounding Ser235 and Ser236 of human Ribosomal protein S6.

Recognizes endogenous levels of human S6 Ribosomal Protein only when phosphorylated at Ser235 and Ser236.

mouse and rat

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.