Immunofluorescence
Immunofluorescent staining of 293T cells were transfected with SARS CoV-2 spike plasmid and stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) (1:1000).
Enzyme-linked Immunoabsorbent Assay
ELISA assay of SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) to SARS-CoV-2 S1-RBD, SARS-CoV-2 S1, SARS-CoV S1, SARS-CoV S1-RBD, MERS-CoV S1-RBD and BSA (BSA: negative control).
Western Blot (Transfected lysate)
Western Blot analysis of 293T cells were transfected with SARS-CoV-2 VLP (Virus-like particle) were stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) at 1:5000 dilution.
Western Blot (Recombinant protein)
Western Blot analysis of different recombinant proteins were stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) at 1:5000 dilution. Lane 1: SARS-CoV-2 S1-RBD, Lane 2: SARS-CoV-2 S1, Lane 3: SARS-CoV S1-RBD, Lane 4: SARS-CoV S1, and Lane 5: MERS-CoV S1-RBD.
- Specifications
Product Description
Mouse monoclonal antibody raised against SARS-CoV-2 S.
Immunogen
SARS-CoV-2 VLP (Virus-like particle).
Host
Mouse
Reactivity
SARS-CoV-2
Form
Liquid
Conjugation
FITC
Purification
Protein A purification
Purity
> 98% (SDS-PAGE).
Isotype
IgG1, kappa
Recommend Usage
ELISA (1:5000-1:20000)
Flow Cytometry
Immunofluorescence(1:500-1:1000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at 4°C for at least 6 months. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This monoclonal antibody is derived from SARS-CoV-2 VLP immune mouse.
- Applications
Immunofluorescence
Immunofluorescent staining of 293T cells were transfected with SARS CoV-2 spike plasmid and stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) (1:1000).Flow Cytometry
Enzyme-linked Immunoabsorbent Assay
ELISA assay of SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) to SARS-CoV-2 S1-RBD, SARS-CoV-2 S1, SARS-CoV S1, SARS-CoV S1-RBD, MERS-CoV S1-RBD and BSA (BSA: negative control).Western Blot (Transfected lysate)
Western Blot analysis of 293T cells were transfected with SARS-CoV-2 VLP (Virus-like particle) were stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) at 1:5000 dilution.Western Blot (Recombinant protein)
Western Blot analysis of different recombinant proteins were stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) at 1:5000 dilution. Lane 1: SARS-CoV-2 S1-RBD, Lane 2: SARS-CoV-2 S1, Lane 3: SARS-CoV S1-RBD, Lane 4: SARS-CoV S1, and Lane 5: MERS-CoV S1-RBD.