Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Serotonin (5-Hydroxytryptamine, 5-HT, Neurotransmitter, Enteramine, Thrombocytin, 3-(?-Aminoethyl) -5-Hydroxyindole, 3-(2-aminoethyl)- 1H-indol-5-ol)

Serotonin (5-Hydroxytryptamine, 5-HT, Neurotransmitter, Enteramine, Thrombocytin, 3-(?-Aminoethyl) -5-Hydroxyindole, 3-(2-aminoethyl)- 1H-indol-5-ol)

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Cat# 301751-500ul

Size : 500ul

Marca : US Biological


301751Serotonin (5-Hydroxytryptamine, 5-HT, Neurotransmitter, Enteramine, Thrombocytin, 3-(?-Aminoethyl) -5-Hydroxyindole, 3-(2-aminoethyl)- 1H-indol-5-ol)

Specifications
Clone TypeMonoclonal
Hostrat
IsotypeIgG
GradeSupernatant
ApplicationsChIP IF IHC
Shipping TempBlue Ice
Storage Temp-20°C

Formaldehyde conjugate of serotonin. Without paraformaldehyde treatment, it cross-reacts in liquid phase with dopamine and tryptamine in hemagglutination tests. The antibody reacts with serotonin in neurons and recognizes serotonergic sites in fixed tissue sections. It does not react with catecholamine-containing neurons in formaldehyde fixed preparations. By immunocytochemistry no cross-reactivity with dopamine and noradrenaline. By red cell agglutination, no cross-reactivity with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine, melatonin

Applications:
Suitable for use in ChIP, Immunohistochemistry and Agglutination. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (FFPE): 1:100-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Mab|Isotype: IgG|Clone No: YC5/45|Host: rat|Concentration: Not Determined |Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with 500ul ddH2O. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution.|Purity: Tissue culture supernatant|Immunogen: Serotonin prepared from culture medium|Specificity: Recognizes Serotonin.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Serotonin prepared from culture medium
Form
Supplied as a lyophilized powder. No preservative added. Reconstitute with 500ul ddH2O. To limit antibody loss or degradation, 1% BSA, 0.02% sodium azide can be added to the suggested first dilution.
Purity
Tissue culture supernatant
Specificity
Recognizes Serotonin.
References
1. Consolazione, A., Milstein, C., Wright, B., and Cuello, A.C. (1981). Immunocytochemical detection of serotonin with monoclonal antibodies. J Histochem Cytochem 29, 1425-1430.