Modified, non-reducing sample buffer for SDS PAGE, delivered as 4x concentrate.

Lithium dodecyl sulfate (LDS) replaces the commonly used sodium dodecyl sulfate (SDS) in the triethanolamine buffered sample buffer. LDS prevents degradation of proteins during sample preparation and heating and stabilizes the pH of the sample during gel run. It does not crystallize at lower temperatures due to significantly better solubility than SDS. Hence, protein stability as well as band resolution is significantly enhanced by cooled gel runs.
For reducing conditions the sample buffer may be supplemented with 10 mM DTT or 5 % β-mercaptoethanol (end concentration 1x sample buffer).
Contains:
Triethanolamine buffered, 40 % glycerol, 4 % Ficoll^® 400, 4 % LDS, 0.025 % phenol red and 0.025 % bromophenol.