Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml. Flow Cytometry. Immunocytochemistry.
Reactivity
Human, Mouse
Antibody Host
Mouse
Isotype
IgG3
Clonality
Monoclonal
Immunogen
Synthetic phosphopeptide conjuagted to KLH
Specificity
This antibody specifically interacts with activated src phosphorylated at Tyr 416 and with src family kinases phosphorylated at the corresponding Tyr in the activation loop. The antibody does not crossreact with the non-phosphorylated form of src nor with unrelated phosphorylation sites.
Buffer
0.5 mg/ml in PBS/0.09% Na-Azide/PEG and Sucrose. Label: Biotin State: Liquid purified IgG fraction.
Purification
Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation
Biotin
Storage
Store the antibody (Aliquote and freeze in liquid nitrogen)at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Src is the best understood member of a family of nine tyrosine kinases regulating cellular responses to extracellular stimuli. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an open, active conformation which allows binding to other proteins via the SH2 and SH3 domain. Activated src phosphorylates a variety of substrates involved in the mitogenic signalling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication, and RNA processing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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