Streptavidin Streptomyces avidinii Recombinant Protein
Cat# TP750175
Size : 1mg
Streptavidin Streptomyces avidinii Recombinant Protein
SKU
TP750175
Purified recombinant protein streptavidin from Streptomyces avidinii, Tag free, expressed in E.coli, 1 mg
| Product Data | |
| Species | Streptomyces avidinii |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | Tag Free |
| Predicted MW | 13.4KDa |
| Concentration | >0.05 µg/µL as determined by spectrophotometrically（A280） method |
| Purity | > 98% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | Lyophilized from a solution containing 20 mM PB, pH 6.5 |
| Bioactivity | > 17 U/mg (one unit binds 1 µg D-biotin) |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -20°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Ambient |
| Reference Data | |
| FAQs |
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| SDS |
| Recombinant Protein Resources |
Citations
Identification and validation of EMT-immune-related prognostic biomarkers CDKN2A, CMTM8 and ILK in colon cancer
Kang, Xie, Zhou et al
BMC Gastroenterol (2022) 22 (1), 190 DOI: 10.1186/s12876-022-02257-2
Kang, Xie, Zhou et al
BMC Gastroenterol (2022) 22 (1), 190 DOI: 10.1186/s12876-022-02257-2
Cited by 33
AI Summary: In this study, OriGene products—including the secondary antibody (SP-9001), horseradish labeled streptavidin (SP-9001), and diaminobenzidine (ZLI-9018)—were used for immunohistochemical staining to validate the expression of prognostic biomarkers CDKN2A, CMTM8, and ILK in colon cancer tissues.
Snippet: were incubated with CDKN2A (concentration 1:200), CMTM8 (concentration 1:50) and ILK (concentration 1:50) antibodies overnight (4℃). Then we stained the tissue sections with the secondary antibody (ORIGENE SP-9001) for 20 min at room temperature. Next, horseradish labeled streptavidin (ORIGENE SP-9001) was utilized to incubate tissues for 15 min. We utilized diaminobenzidine (ORIGENE ZLI-9018);- ...
Co-Overexpression of GRK5/ACTC1 Correlates With the Clinical Parameters and Poor Prognosis of Epithelial Ovarian Cancer
Liu, Lv, Lin et al
Front Mol Biosci (2021) 8, 785922 DOI: 10.3389/fmolb.2021.785922
Liu, Lv, Lin et al
Front Mol Biosci (2021) 8, 785922 DOI: 10.3389/fmolb.2021.785922
Cited by 9
AI Summary: In this study, OriGene products—specifically the GRK5 (Cat# RC209307) and ACTC1 (Cat# RC203682) expression plasmids—were used to investigate the expression and prognostic significance of GRK5 and ACTC1 in epithelial ovarian cancer tissues.
MicroRNA‑218 inhibits the malignant phenotypes of glioma by modulating the TNC/AKT/AP‑1/TGFβ1 feedback signaling loop
Dang, Zhang, Tian et al
Int J Mol Med (2021) 48 (5) DOI: 10.3892/ijmm.2021.5038
Dang, Zhang, Tian et al
Int J Mol Med (2021) 48 (5) DOI: 10.3892/ijmm.2021.5038
Cited by 13
AI Summary: OriGene’s goat anti-rabbit antibody (TA130023) and goat anti-mouse antibody (TA130004) were used as secondary antibodies in western blot experiments to detect protein expression changes underlying the tumor-suppressive effects of miR-218 in glioma cells.
Snippet: … TA130023;OriGene Technologies, Inc.) or goat anti‑mouse antibody (1:3,000;TA130004;OriGene Technologies, Inc.), for 2 h at 37˚C, the Western Bright ECL detection system (Advansta, Inc.) was used to visualize the immunoblotting signals
IL-6 induces tumor suppressor protein tyrosine phosphatase receptor type D by inhibiting miR-34a to prevent IL-6 signaling overactivation
Zhang, Wang, Qin et al
Mol. Cell. Biochem. (2020)
Zhang, Wang, Qin et al
Mol. Cell. Biochem. (2020)
Cited by 11
AI Summary: OriGene’s ZSGB-BIO secondary antibody and streptavidin-biotin solution were used for immunohistochemical detection of IL-6, PTPRD, and p-STAT3 in tissue sections, enabling analysis of protein expression in the study.
Snippet: The sections were then incubated with polyclonal antibodies against IL-6 (1:100), PTPRD (1:100), and p-STAT3 (1:100), followed by a secondary antibody (OriGene ZSGB-BIO, Shanghai, China) and streptavidin-biotin solution (OriGene ZSGB-BIO)
Combination of the ginsenosides Rb3 and Rb2 exerts protective effects against myocardial ischemia reperfusion injury in rats
Liu, Jiang, Fu et al
Int. J. Mol. Med. (2019) 45 (2), 519-531 DOI: 10.3892/ijmm.2019.4414
Liu, Jiang, Fu et al
Int. J. Mol. Med. (2019) 45 (2), 519-531 DOI: 10.3892/ijmm.2019.4414
AI Summary: In this study, the avidin-biotin-streptavidin complex from OriGene Technologies, Inc. was used for immunohistochemical detection of protein expression in myocardial tissue sections, contributing to the analysis of cardioprotective effects of ginsenosides against myocardial ischemia reperfusion injury in rats.
Snippet: temperature. Subsequently, sections were washed prior to development with avidin-biotin-streptavidin complex (OriGene Technologies, Inc.) at 30°C for 20 min and diami-nobenzidine chromogen for 15 min at room temperature;- temperature. Subsequently, sections were washed prior to development with avidin‑biotin‑streptavidin complex (OriGene Technologies, Inc.) at 30˚C for 20 min and diami‑ nobenzi...
MYH9 overexpression correlates with clinicopathological parameters and poor prognosis of epithelial ovarian cancer
Liu, Yi, Deng et al
Oncol Lett (2019) 18 (2), 1049-1056 DOI: 10.3892/ol.2019.10406
Liu, Yi, Deng et al
Oncol Lett (2019) 18 (2), 1049-1056 DOI: 10.3892/ol.2019.10406
AI Summary: In this study, OriGene's MYH9 polyclonal antibody (Cat# TA890003) was used for immunohistochemical detection of MYH9 expression in ovarian cancer tissues, enabling the researchers to evaluate the association between MYH9 overexpression and poor prognosis in epithelial ovarian cancer.
Showing 1-6 of 14 papers.
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