SW-1463
Marca : CLS Cell Lines Service
SW-1463 Cells
General information
Organism | Human |
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Tissue | Rectum |
Disease | Rectal adenocarcinoma |
Applications | 3D culture, Cancer research |
Synonyms | SW1463, SW 1463 |
Characteristics
Age | 66 years |
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Gender | Female |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SW-1463 (Cytion catalog number 300623) |
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Biosafety level | 1 |
Expression / Mutation
Surface antigens | Blood type A, Rh + |
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Protein expression | keratin |
Antigen expression | carcinoembryonic antigen (CEA) |
Isoenzymes | ES-D, 1, G6PD, B, PEP-D, 1, PGD, A, PGM1, 1, PGM3, 1-2 |
Tumorigenic | Yes, in nude mice |
Ploidy status | hypertriploid |
Karyotype | 2n=46 |
Handling
Culture Medium | Leibovitz's L-15, w: 2.0 mM L-Glutamine, 0.55 g/L NaHCO3 (We do not supply this product; please consider other suppliers. Please let us know if you need further assistance) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 11,12 D13S317: 12,13 D16S539: 11 D5S818: 13,14 D7S820: 9 TH01: 6,7 TPOX: 8,11 vWA: 16 D3S1358: 16,17 D21S11: 30,31.2 D18S51: 18 Penta E: 17 Penta D: 9,12 D8S1179: 11,15 FGA: 23,28 D6S1043: 12,18 D2S1338: 17,18 D12S391: 17 D19S433: 14,15 |