Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

TCEP hydrochloride [51805-45-9]

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Cat# NB-64-38935-1g

Size : 1g

Marca : Neo Biotech

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TCEP hydrochloride (Synonyms: Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl)

Catalog No. T7087 Copy Product Info

Purity: 99.93%

TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a trialkylphosphine that selectively reduces proteolysis. TCEP hydrochloride is a non-thiol-reducing agent that promotes the binding of NF-κB-DNA.

TCEP hydrochloride

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Synonyms Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl

Cas No. 51805-45-9

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Purity:99.93%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

TCEP hydrochloride AI Summary

TCEP hydrochloride shows no significant inhibitory activity against HDAC1 and HDAC6 enzymes. Additionally, it does not exhibit significant growth inhibition (GI50 > 50000.0 nM) on human A549, MCF7, and WI38 cells after 96 hours, as determined by the sulphorhodamine B assay..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a trialkylphosphine that selectively reduces proteolysis. TCEP hydrochloride is a non-thiol-reducing agent that promotes the binding of NF-κB-DNA.
Targets&IC50	MCF-7 cells:> 50 μM (GI50), WI-38 cells:> 50 μM (GI50), NS3/4A:12.8 μM, A549 cells:> 50 μM (GI50)
In vitro	METHODS: Human hepatocellular carcinoma cells HepG2 were treated with TCEP hydrochloride (100-400 µM) for 3 days, and cell viability was detected by MTT assay. RESULTS: The viability of HepG2 was significantly reduced to 25.68% and 70.92% after treatment with 200 and 400 µM TCEP for 3 days, whereas the lowest concentration of TCEP (100 µM) did not significantly reduce the viability of HepG2 (3.44%). [1] METHODS: Human peripheral blood mononuclear cell PBMCs were treated with TCEP hydrochloride (0.5-1 mM) for 24 h. Morphological and necrotic changes were assessed by Hoechst 33342/PI staining. RESULTS: Necrosis was observed in cells treated with 0.5 mM of TCEP. [2]
In vivo	METHODS: To investigate the neurotoxicity and its mechanism in mice, TCEP hydrochloride (10-100 mg/kg) was administered by gavage to Kunming mice once a day for 30 days. RESULTS: Compared with the control group, the high dose TCEP group showed a significant decrease in water intake and a significant increase in liver and spleen organ indices. In the water maze test, the escape latency of TCEP-exposed mice was longer than that of the control group, and the total swimming course of the high-dose TCEP group was higher than that of the control group, and the swimming time of the target quadrant was significantly shorter.TCEP exposure may cause neurotoxicity by increasing thyroid hormones and inducing oxidative damage in mice. [3]
Synonyms	Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl

Chemical Properties

Molecular Weight	286.65
Formula	C₉H₁₆ClO₆P
Cas No.	51805-45-9
Smiles	Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O
Relative Density.	1.041 g/cm3 at 25℃

Storage & Solubility Information

Storage

Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 252.5 mg/mL (880.87 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 2.75 mg/mL (9.59 mM), Solution.

5% DMSO+95% Saline: 1.45 mg/mL (5.06 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.4886 mL	17.4429 mL	34.8857 mL	174.4287 mL
5 mM	0.6977 mL	3.4886 mL	6.9771 mL	34.8857 mL
10 mM	0.3489 mL	1.7443 mL	3.4886 mL	17.4429 mL
20 mM	0.1744 mL	0.8721 mL	1.7443 mL	8.7214 mL
50 mM	0.0698 mL	0.3489 mL	0.6977 mL	3.4886 mL
100 mM	0.0349 mL	0.1744 mL	0.3489 mL	1.7443 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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