TransTaq® DNA Polymerase High Fidelity (HiFi) (with 2.5 mM dNTPs)

Cat# AP131-12

Size : 500units

Marca : TransGen Biotech

Contatta il distributore locale :


Telefono : +1 850 650 7790

  • AP131-01.JPG
Product Details

TransTaq® DNA Polymerase High Fidelity (TransTaq®HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.

TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.

Applications
• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAP131-01/11AP131-02/12AP131-03/13
TransTaq® HiFi DNA Polymerase250 U×1500 U×1500 U×6
10×TransTaq® HiFi Buffer I1.2 ml×11.2 ml×11.2 ml×6
10×TransTaq® HiFi Buffer II1.2 ml×11.2 ml×11.2 ml×6
2.5 mM dNTP-/400 μl×1-/800 μl×1-/800 μl×6
10×GC Enhancer200 μl×1400 μl×11 ml×1
6×DNA Loading Buffer500 μl×11 ml×11 ml×2


Citations

Li P, Du J, Goodier J L, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion[J]. Nucleic acids research, Nucleic Acids Res. 2017, 45(8):4619-4631.

Che L H, Zhang SQ, Li Y, et al. Genome-wide survey of nuclear protein-coding markers for beetle phylogenetics and their application in resolving both deep and shallow-level divergences[J]. Molecular Ecology Resources, 2017, 17(6):1342.

You CX , Zhao Q , Wang XF , et al. A dsRNA-binding protein MdDRB1 associated with miRNA biogenesis modifies adventitious rooting and tree architecture in apple[J]. Plant Biotechnology Journal, 2014, 12(2):183-192.

Gu P , Yang F , Kang J , et al. One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli[J]. Microbial Cell Factories, 2012, 11(1):30.

Wang G H , Sun B F , Xiong T L , et al. Bacteriophage WO Can Mediate Horizontal Gene Transfer in Endosymbiotic Wolbachia Genomes[J]. Frontiers in Microbiology, 2016, 7:1867.

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