Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Tyrosine Hydroxylase antibody

Tyrosine Hydroxylase antibody

Stampa Stampa
Entra

Cat# GTX113016-25ul

Size : 25ul

Marca : Genetex

Contatta il distributore locale :


Telefono : +1 850 650 7790

Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016
Anti-Tyrosine Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX113016
Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016
Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016
Anti-Tyrosine Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Zebrafish, Vole, Calf
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX113016 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

NT2D1 , mouse brain

Predict Reactivity

Bovine, Dog, Chicken, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952230

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine hydroxylase , DYT14 , DYT5b , TYH

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Database

DATA IMAGES

Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016

GTX113016 IHC-Fr Image

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Tyrosine Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX113016

GTX113016 IHC-P Image

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016

GTX113016 IHC-Fr Image

Immunohistochemical analysis of Rat hindlimb pad skin tissue (paraformaldehyde-fixed frozen sections), using Tyrosine Hydroxylase(GTX113016) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016

GTX113016 IHC-Fr Image

Immunohistochemical analysis of Rat hindlimb pad skin tissue (paraformaldehyde-fixed frozen sections), using Tyrosine Hydroxylase(GTX113016) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016

GTX113016 WB Image

Tyrosine Hydroxylase antibody detects TH protein by western blot analysis.
A. 30 μg NT2D1 whole cell lysate/extract
B. 30 μg PC-3 whole cell lysate/extract
C. 30 μg U87-MG whole cell lysate/extract
D. 30 μg SK-N-SH whole cell lysate/extract
7.5% SDS-PAGE
Tyrosine Hydroxylase antibody (GTX113016) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tyrosine Hydroxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113016

GTX113016 ICC/IF Image

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:400.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016

GTX113016 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:1000.

Anti-Tyrosine Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX113016

GTX113016 IHC-P Image

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:2500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016

GTX113016 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016

GTX113016 WB Image

Zebrafish tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:500.

Application Reference

Wu CY et al. Biology (Basel) 2023; 12 (10) Klotho Null Mutation Indirectly Leads to Age-Related Lacrimal Gland Degeneration in Mutant Mice.
N Ikeda et al. Neuroscience 2023; S0306-4522 (23) : 00337-8 Chemogenetic Activation of Oxytocin Neurons Improves Pain in a Reserpine-induced Fibromyalgia Rat Model
Reactivity : Rat
Yamamoto S et al. Front Immunol 2022; 13 : 960036 <i>In Vitro</i> Generation of Brain Regulatory T Cells by Co-culturing With Astrocytes.
Lasse Reimer et al. Sci Rep 2022; 12 (1) : 3737 Low dose DMSO treatment induces oligomerization and accelerates aggregation of α-synuclein.
Application : IHC-Fr
Reactivity : Mouse
Di Nisio A et al. Environ Int 2022; 158 : 106982 Impairment of human dopaminergic neurons at different developmental stages by perfluoro-octanoic acid (PFOA) and differential human brain areas accumulation of perfluoroalkyl chemicals.
Reactivity : Human
Submit a Reference

REVIEW

Tyrosine Hydroxylase antibody Cat. No. GTX113016

Rating
( Average 5 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 2 users reviews)
Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the 2022 in Front Immunol. PMID: 35911740
Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2017. PMID: 28818835
Application Reference
Wu CY et al. Biology (Basel) 2023; 12 (10) Klotho Null Mutation Indirectly Leads to Age-Related Lacrimal Gland Degeneration in Mutant Mice.
N Ikeda et al. Neuroscience 2023; S0306-4522 (23):00337-8 Chemogenetic Activation of Oxytocin Neurons Improves Pain in a Reserpine-induced Fibromyalgia Rat Model
Reactivity : Rat
Yamamoto S et al. Front Immunol 2022; 13:960036 <i>In Vitro</i> Generation of Brain Regulatory T Cells by Co-culturing With Astrocytes.
Lasse Reimer et al. Sci Rep 2022; 12 (1):3737 Low dose DMSO treatment induces oligomerization and accelerates aggregation of α-synuclein.
Application : IHC-Fr
Reactivity : Mouse
Di Nisio A et al. Environ Int 2022; 158:106982 Impairment of human dopaminergic neurons at different developmental stages by perfluoro-octanoic acid (PFOA) and differential human brain areas accumulation of perfluoroalkyl chemicals.
Reactivity : Human
Wejdane El Manaa et al. Autophagy 2021; 17 (12):4363-4385 Transcription- and phosphorylation-dependent control of a functional interplay between XBP1s and PINK1 governs mitophagy and potentially impacts Parkinson disease pathophysiology.
Application : WB
Reactivity : Human
Song Y et al. Neurosci Bull 2021; (Epub) Dopaminergic Neurons in the Ventral Tegmental-Prelimbic Pathway Promote the Emergence of Rats from Sevoflurane Anesthesia.
Application : IHC-P
Reactivity : Rat
Fede C et al. Sci Rep 2021; 11 (1):12623 Evidence of a new hidden neural network into deep fasciae.
Application : IHC-P
Reactivity : Mouse
Kawabe M et al. Neuropeptides 2021; 87:102136 Ninjinyoeito improves anxiety behavior in neuropeptide Y deficient zebrafish.
Wu J et al. Oxid Med Cell Longev 2020; 2020:8735249 The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson's Disease.
Wu CY et al. Brain Stimul 2020; 13 (3):786-799 A preliminary study of Parkinson's gene therapy via sono-magnetic sensing gene vector for conquering extra/intracellular barriers in mice.
Application : WB
Reactivity : Mouse
Santana Y et al. J Clin Med 2019; 8 (2) Understanding the Mechanism of Antidepressant-Related Sexual Dysfunction: Inhibition of Tyrosine Hydroxylase in Dopaminergic Neurons after Treatment with * but Not with Agomelatine in Male Rats.
Reactivity : Rat
Li Y et al. Oxid Med Cell Longev 2018; 2018:2917981 The Protective Role of Brain CYP2J in Parkinson's Disease Models.
Li Y et al. Oxidative Medicine and Cellular Longevity 2018; (Epub) The Protective Role of Brain CYP2J in Parkinson’s Disease Models.
Application : IHC-P
Reactivity : Rat
Park CH et al. J Vet Med Sci 2018; (Epub) Cervical ganglioneuroblastoma in a new born Japanese Black calf.
Application : IHC-P
Reactivity : Calf
Zhang L et al. EMBO Mol Med 2017; (Epub) Src-dependent phosphorylation of μ-opioid receptor at Tyr336 modulates opiate withdrawal.
Application : IHC-Fr
Reactivity : Mouse
Arai A et al. Behav Brain Res 2016; 315:83-93 A single prolonged stress paradigm produces enduring impairments in social bonding in monogamous prairie voles.
Application : IHC-Fr
Reactivity : Vole
Ma C et al. Life Sci 2016; 144:113-20 Pre-administration of BAX-inhibiting peptides decrease the loss of the nigral dopaminergic neurons in rats.
Application : IHC-P
Reactivity : Rat
Xiao JJ et al. Oxid Med Cell Longev 2015; 2015:618631 Transplanted Neural Stem Cells: Playing a Neuroprotective Role by Ceruloplasmin in the Substantia Nigra of PD Model Rats?
Application : WB, IHC-Fr
Reactivity : Rat
Thomas MG et al. Cell Death Dis 2013; 4:e669 Nicotinamide N-methyltransferase expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Si può anche essere interessati nei seguenti prodotti:



Cat#
Descrizione
Cond.
Priced
GTX103261-25ul
Calretinin antibody
 25ul 
GTX103261-100ul
Calretinin antibody
 100ul 
GTX113016-100ul
Tyrosine Hydroxylase antibody
 100ul