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Product Description
This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF ® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.
Catalog number key for antibody number 1687, Anti-VEGF (VG76e)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified,
BSA-free | N/A | N/A | N/A | |
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
Technology
Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
Product Attributes
Antibody reactivity (target)
VEGF
Molecular weight
19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)
Synonyms
MVCD1; Vascular Endothelial Growth Factor A (VEGF-A); Vascular Permeability Factor (VPF); VEGFA
Immunogen
Human VEGF189 recombinant protein
Antibody target cellular localization
Plasma membrane, Secreted (extracellular)
Expected antibody applications
Functional studies (published for clone), IHC (FFPE) (published for clone), WB (published for clone)
Antibody application notes
ELISA: For coating, order Ab without BSA; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined., Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
Positive control
Tumor cells in astrocytomas, breast or ovarian carcinomas.
Shipping condition
Room temperature
Storage Conditions
Note: store BSA-free antibodies at -10 to -35°C, Store at 2 to 8 °C, Protect fluorescent conjugates from light
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression
Endothelial cells
Documents, Protocols, SDS and COA
Datasheet
Datasheet Download
COARequest the Certificate of Analysis
References
Note: References for this clone sold by other suppliers may be listed for expected applications. - Endocrinology (2000) 141(3): 995-1000. (WB, functional studies)
- J Vet Diag Invest (2010) 22(1):105-108. (IHC, FFPE)
Citations
Note: Note: Listed references are for this antibody clone sold by Biotium and other suppliers.
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